Adjusting selectivity in micellar electrokinetic capillary. Micellar electrokinetic chromatography mekc is a chromatography technique used in analytical chemistry. Vitha, timothy urness, and thomas marrinan department of chemistry and department of mathematics and computer science, drake university, 2507 university. Separation of benzene, toluene, ethylbenzene, and xylenes. Micellar electrokinetic chromatography mekc is a highly efficient capillary. Micellar electrokinetic chromatography technische universiteit. Exceeding 5000fold concentration of dilute analytes in. Determination of caffeine and associated compounds in food.
This method can provide highly efficient separation for neutral and hydrophobic compounds. A micellar electrokinetic capillary chromatographic method, a variant of capillary electrophoresis, was developed for the detection and measurement of pirfenidone and 5carboxypirfenidone by direct injection of plasma from patients under treatment for idiopathic pulmonary fibrosis. Eof consists of results from the presence of negatively charged walls on capillary surface i. A micellar electrokinetic capillary chromatographic mekc method was developed for the two major polymethoxylated flavones, nobiletin and tangeretin, using a fusedsilica opentubular capillary in a 70% methanol extract from citrus albedo tissue. Separation of retinoids by micellar electrokinetic. This separation is achieved by the addition of ionic micelles to the electrophoretic medium. Pdf principles of micellar electrokinetic capillary chromatography.
A method is described for quantitating caffeine, theobromine, theophylline, paracetamol, propyphenazone, acetylsalicylic acid, salicylic acid, and codeine phosphate in corresponding real samples of food, beverages, natural products, pharmaceuticals, and cosmetic preparations by micellar electrokinetic capillary chromatography. Validated micellar electrokinetic capillary chromatography. Preparation and evaluation of fused silica capillary. Capillary electrophoresis ce is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro and nanofluidic channels. Separation of dansyl dlamino acids by micellar electrokinetic capillary chromatography with and without cyclodextrins using pace system 2000. Principles of micellar electrokinetic capillary chromatography. Optimization of the separation of phenolic compounds by micellar electrokinetic capillary chromatography. Pdf principles of micellar electrokinetic capillary. A simple and sensitive cyclodextrin micellar electrokinetic capillary chromatography cdmekc method with uv detection was developed and validated for the determination of vancomycin vcm in serum. Micellar electrokinetic chromatography wiley online library. Determination of vancomycin in human serum by cyclodextrin. Chromatography, supercriticalfluid chromatography, highpressure liquid chromatography, and micellar electrokinetic capillary chromatography systems andrew r. Mekc can be performed by adding an ionic micelle to the running solution of ce without modifying the instrument. Micellar electrokinetic capillary chromatography, a hybrid method that combines chromatographic and electrophoretic separation principles, extends the.
Micellar electrokinetic capillary chromatography mekc is a variation of ce in which separation of both charged and neutral compounds is accomplished on the basis of differential partitioning in a migrating micelle phase. Micellar electrokinetic chromatography mekc is a mode of ekc in which surfactants micelles are added to the buffer solution. Reversed phase high performance liquid chromatography hplc and micellar electrokinetic capillary chromatography mekc successfully separated mixtures of arsine compounds and explosives. Pdf optimization of the separation of phenolic compounds. Very often, ce refers to capillary zone electrophoresis cze, but other electrophoretic techniques including capillary gel electrophoresis cge, capillary isoelectric focusing cief, capillary isotachophoresis and micellar. Determination of total ascorbic acid in beers, wines, and. Preparation and evaluation of fused silica capillary columns for gas chromatography and micellar electrokinetic chromatography. A monomolecular pseudostationary phase for micellar electrokinetic capillary chromatography. Because only brief discussion on some aspects of mekc was described in this article, it is. Pdf fundamentals of micellar electrokinetic chromatography. Electrokinetic chromatography ekc is a family of electrophoresis techniques named after electrokinetic phenomena, which include electroosmosis, electrophoresis, and chromatography. Gradient elution for micellar electrokinetic capillary. Micellar electrokinetic chromatography mekc is a highly efficient separation method. Fast method for quantitative determination of methylene.
Simultaneous determination of atorvastatin and ezetimibe. Micellar electrokinetic capillary chromatography of glucosinolates and desulfoglucosinolates with a cationic surfactant. A rapid micellar electrokinetic capillary chromatographic method is described for the determination of lascorbic acid in beer, wine, and fruit juice we use cookies to enhance your experience on our website. As a result, in 1984, micellar electrokinetic capillary chromatography mekc was developed to separate neutral compounds 2. Communications separation of metal bis2hydroxyethyl. A micellar electrokinetic capillary chromatography mecc method when compared to free solution capillary electrophoresis cze was shown to offer improved selectivity and resolution for the separation of uv. Capillary electrophoresis, micellar electrokinetic, capillary chromatography, pharmaceutical analysis introduction capillary electrophoresis ce is an instrumental evolution of traditional electrophoretic techniques, where separation occurs in fusedsilica capillaries and involves application of high voltages across buffer filled. The separation mechanism in mekc is based on differences in distribution equilibria between an aqueous phase and a micellar phase. Determination of saikosaponins by micellar electrokinetic.
Micellar electrokinetic chromatography an overview. Electrophoresis was first developed in the 1930s by arne tiselius, who separated serum proteins using their charge differences. Micellar electrokinetic capillary chromatography, a hybrid method that combines chromatographic and electrophoretic separation principles. Micellar electrokinetic capillary chromatography mecc experimental conditions were applied to 12 samples of methylxanthinecontaining infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. Micellar electrokinetic capillary chromatography robin. Separation of arsines and trinitrotoluene by reversed. The method developed was based on micellar electrokinetic chromatography meck. Micellar electrokinetic chromatography micellar electrokinetic chromatography mekc is a hybrid of electrophoresis and chromatography which uses surfactants in the running buffer, thus making the separation of neutral species possible. It is a modification of capillary electrophoresis ce, extending its functionality to neutral analytes, 1 where the samples are separated by differential partitioning between micelles pseudostationary phase and a surrounding aqueous buffer solution. Micellar electrokinetic chromatography mekc, a separation mode of capillary electrophoresis ce, has enabled the separation of electrically neutral analytes.
Electrophoresis for bioapplications dna, rna, and protein. Preparation and evaluation of fused silica capillary columns for gas chromatography and micellar electrokinetic chromatography citation for published version apa. The ce results suggested that both cnds are highly hydrophilic on anionic cnds and lesscharged cnds. Experimental conditions such as buffer concentration and ph, surfactant. Micelles are aggregates of surfactant molecules that form when a surfactant is added to. Anionic and lesscharged cnds were found in bgcnd and gcnd by the ce investigations. In order to achieve serum proteins solubilization and coelution with micelle, 100 mm sds was used in this study.
Special issue dedicated to our editor in chief, frantisek svec, on the occasion of his 75th birthday virtual special issue molecular imprinting in separation science virtual special issue. A simple and validated micellar electrokinetic chromatographic method is described for the determination of 5hydroxymethylfurfural hmf. Micellar electrokinetic chromatography is a modified version of capillary electrophoresis which is used to. Quantification of nobiletin and tangeretin in citrus by. Molecules free fulltext determination of vancomycin. Abstract in this report, we introduce a new micelle modifier useful to alter selectivity in micellar electrokinetic capillary chromatography mecc. Journal of high resolution chromatography 1992, 15 11, 756762. In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed. Column efficiency in micellar electrokinetic capillary. Micellar electrokinetic capillary chromatography of.
Novel pseudostationary phase for micellar electrokinetic capillary chromatography. Ekc offers diverse advantages over other techniques including high efficiency, technical simplicity, applicability to most analytes. Determination of urinary hippuric acid by micellar electrokinetic capillary chromatography article in journal of chromatography b 7932. Determination of urinary hippuric acid by micellar. Micellar electrokinetic capillary chromatography mecc of. Mekc is a variation of ce in which separation of both charged and neutral compounds is accomplished on the. Optimization of the chiral resolution of metyrosine by capillary electrophoresis andor micellar electrokinetic capillary chromatography mohamed m. Micellar electrokinetic capillary chromatography 4. Electrokinetic chromatography ekc, a mode of capillary electrophoresis, is a group of analytical separation techniques named after electrokinetic phenomena that include electroosmosis, electrophoresis, and chromatography. The complexes were formed by pre capillary reaction of the metal ions with an excess of the ligand and then separated using a 100 mm borate buffer ph 9. This technique was used instead of capillary zone electrophoresis cze since mb is adsorbed on the capillary wall in ph above 5. Infinite elution range in micellar electrokinetic capillary chromatography using a nonionicanionic mixed micellar system.
Micellar electrokinetic chromatography springerlink. Another advantage of the use of meck is a very short analysis time while using inverse polarity. Publishers pdf, also known as version of record includes final. During the experiment, the capillary is filled with the electrolyte solution and the sample to be. Analysis of urinary pseudouridine by micellar electrokinetic capillary chromatography m d evans, d perrett1, j lunec and k e herbert from the division ofchemical pathology, centre for mechanisms ofhuman toxicity, hodgkin building, po box 8, university ofleicester, lancaster road, leicester lei 9hn, and the. Mekc, a capillary electromigration separation technique for both charged. Capillary electrophoretic characterization of water. Micellar electrokinetic capillary chromatography mekc mekc is a separation technique that is based on solutes partitioning between micelles and the solvent. Micellar electrokinetic chromatography is a modified version of capillary electrophoresis which is used to separate a wide range of molecules, from inorganic ions to large biopolymers. The under lying separation mechanism of mekc is that ana lytes have different partition coefficients between the. Hplc separation was accomplished with a methanolwater gradient and detection with an uvdad detector. A rapid and sensitive micellar electrokinetic capillary chromatography method with uv photodiodearray detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Elsevier journal of chromatography a, 759 1997 193201 journal of chromatography a determination of saikosaponins by micellar electrokinetic capillary chromatography youzung hsieh, hsiya huang department of applied chemistry, national chiao tung university, 1001 tahsueh road, hsinchu 30050, taiwan. Journal of high resolution chromatography 1992, 15 4, 271275.
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